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KMID : 0352720090330030206
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Journal of Ginseng Research
2009 Volume.33 No. 3 p.206 ~ p.211
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The Effect of Haliotidis Concha on the Growth and Ginsenoside Biosynthesis of Korean Ginseng Hairy Root
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Jung Dae-Young
Kim Yu-Jin
Shim Ju-Sun
Lee Jung-Hye
Jung Seok-Kyu
Kim Se-Young
Yang Deok-Chun
In Jun-Gyo
Lee Bum-Soo
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Abstract
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In order to investigate the effects of elicitors on the growth and ginsenoside biosynthesis of ginseng hairy roots, we treated Panax ginseng hairy root with various concentrations of Haliotidis concha according to different time course. Haliotidis concha supplement increased the biomass and ginsenoside accumulation at 10 ㎎/L concentration. The growth rate of hairy root under a lighter concentration was greater than hairy root treated with a denser concentration. The highest content and productivity of ginsenosides appeared at 2 weeks after the treatment of 10 ㎎/L Haliotidis concha. The gene expression of squalene synthase, squalene epoxidase, dammarenediol synthase, cycloartenol synthase, β-amyrin synthase in hairy roots of ginseng were examined by RT-PCR. The Haliotidis concha treatment resulted in the obvious accumulation of the mRNA of triterpene biosynthesis in Panax ginseng hairy root as compared with the control. In this study, Haliotidis concha acts as a kind of elicitor for the production of ginsenosides.
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KEYWORD
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elicitor, Haliotidis concha, Panax ginseng hairy root, RT-PCR
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